Among those attending the event were TDs Maureen O’Sullivan and Sean Haughey, as well as Dublin city councillors Larry O’Toole and Dermot Lacey.
The group paraded from the GPO to 16 Moore Street, where the surrender order was signed by the rebellion leadership 94 years ago.
Leading the marchers was a colour party dressed in the uniforms of the Irish Citizen’s Army and Irish Volunteers.
The recently discovered original text of the surrender notice was read out.
Among those in attendance was the great grandson of Citizen Army leader James Connolly.
He called upon the Government to live up to commitments to protect the historic buildings which have national monument status.
Minister for the Environment John Gormley has previously pledged to protect the site, however, he was unable to take up an invitation to attend.
Following speeches by relatives of those who took part in the 1916 fighting the commemoration concluded with white lilies being laid outside the Moore Street building.