Toronado produced a brilliant performance to win the race 12 months ago and is among a field of just five runners.
Since his narrow defeat in the 2000 Guineas at Newmarket in early May, the John Gosden-trained Kingman has claimed hugely impressive victories in the Irish Guineas and the St James’s Palace Stakes at Royal Ascot to confirm himself the leading three-year-old miler in Britain.
Toronado made a winning return to action in the Queen Anne at the Royal meeting but as he is a year older than his major rival, he must concede 8lb in the latest edition of the ’duel on the Downs’.
Aidan O’Brien sends two challengers on the trip from Ireland in Darwin and War Command.
Darwin looks to have his work cut out having been beaten in the Group Three Minstrel Stakes at the Curragh less than a fortnight ago, while War Command won the Dewhurst as a juvenile, but has been well beaten in three starts this year.
The field is completed by another three-year-old in Charlie Appleby’s Godolphin runner Outstrip.
He finished off well to finish third behind Kingman at Royal Ascot, but it would be a major surprise if he were to turn the tables.
Thistle Bird remains on course to bid for a second successive Group One victory after featuring amongst 10 fillies and mares confirmed for the Markel Insurance Nassau Stakes at Glorious Goodwood on Saturday.
Having claimed the Princess Elizabeth Stakes at Epsom for a second time, Roger Charlton’s six-year-old landed her first success at the highest level with a brilliant display under George Baker in last month’s Pretty Polly Stakes at the Curragh.
John Gosden has won the last two renewals of the Nassau with The Fugue and Winsili and has two representatives in contention this time in Sultanina and Eastern Belle.
Sultanina found only stable companion Pomology too strong in the Lancashire Oaks earlier this month, while the three-year-old Eastern Belle was last seen claiming a Listed prize at Newbury.
Aidan O’Brien has entered Pretty Polly runner-up Venus De Milo, as well as Palace.
Mango Diva, Amazing Maria, Lustrous and Madame Chiang are also in contention.
There is also a potential French challenger in Narniyn, who won four races on the spin before finishing fourth against the boys in the Grand Prix de Saint-Cloud.
Georges Rimaud, French racing manager for Narniyn’s owner, the Aga Khan, said the filly is an intended runner in the Nassau.
Rimaud said: ““I think she’ll enjoy the better ground and we are looking forward to seeing her run. I think this is where she belongs.”