Harlequins were beaten 2-0 by Railway in Dublin.
However, with the home leg next weekend, they have a chance of going through.
They will be disappointed to be two down as they played some excellent hockey, but late goals from Karen Clarke, following a penalty corner, and Sinéad Dooley turned the tie in Railway’s favour. Harlequins will be hoping to have Jan Dillon back in defence for the return tie.
They missed her influence in defence. She was missing through a serious head injury.
And despite her willingness to play, medical advice may also see her miss the return leg.
Church of Ireland were at home to Ulster champions Pegasus in the first leg and lost out 5-0.
They lost keeper Lizanne Hourihan late in the week through injury and had to call in teenager Zoe Cremin.
Despite her age, she played well overall, and were it not for some great saves the scoreline could have been a lot worse.
Pegasus took the lead after seven minutes when captain Arlene Boyles scored with a direct shot from a penalty corner and she made it 2-0 after 20 minutes, again from a penalty corner.
A minute later it was 3-0 when Claire McMahon was on target following a sweeping move down the wing involving several players.
In between, Cremin had made good saves from Boyles and Claire McMahon to deny Pegasus.
The second half was a much more even affair with Deirdre Casey and Claire Hickey going close for Church of Ireland.
They looked like holding the score at 3-0 but luck wasn’t on their side as Pegasus were awarded a penalty stroke in the 63rd minute and Boyles made no mistake from the penalty spot to make it 4-0.
Two minutes later Sarah Wilkinson made it 5-0 for the visitors.
With five away goals, it is mission impossible for Church of Ireland as they head to Ulster next weekend, but their young players will have learned a lot from this tie.