The 39-year-old X-Men actor had been tipped as a favourite to land the storied role and is rumoured to be on a shortlist of actors in the running to take over from Daniel Craig, 48.
However, Fassbender told the December issue of GQ the film franchise needed a new spin as Craig had already excelled in 007’s tuxedo and that nobody of Craig’s age could improve the Spectre star’s performance.
Asked whether he would sign up as Bond, the Kerry-raised star said: “To be honest, no.
“As an acting role, I think Daniel has done such a cracking job in this age group... I think the franchise needs something new.”
Fassbender suggested an actress should play “Jane Bond” or a younger actor, such as Unbroken star Jack O’Connell, 26, could take the legendary spy back to his roots.
“What about we start the film back in Sandhurst, Army training rather than on a yacht, or he’s in the Middle East on an op and gets thrown in the brig for insubordination? He’s going to get court-martialled... I have thought about it a lot,” he said.
Fassbender said he is a huge fan of the Bond films.
“I just love Bond. Doesn’t everyone? I grew up with him,” he said.
“It’s always a fun conversation to have. But Bond should be someone in their early 20s ... I always thought Bond should be British, but let’s get an American in or maybe someone like Jack O’Connell would be good?
“Or even better, how about Jane Bond? A woman. One thing is for sure, it won’t be anyone on the bookies’ lists. It never is.”
Luther star Idris Elba, 44, The Night Manager’s Tom Hiddleston, 35, Poldark’s Aidan Turner, 33, Welsh actor Luke Evans, 37, Victoria star Tom Hughes, 30, and War And Peace’s James Norton, 31, have all been tipped for the role.
Fassbender also voiced his opposition to Republican presidential candidate Donald Trump, calling him part of the problem.
“I think people are angry and that’s why Trump got such a huge following,” he told GQ. “They feel disenfranchised and this is their way to strike back. But is he the answer they are looking for?
“Feels to me like he’s someone who got us into this mess in the first place, with the bankers and so on — he’s just part of the problem.”