Aer Arann will fly twice a day from Kerry to Dublin returning mid-morning and, again, at 7 pm.
The schedule has been welcomed by tourist and business travellers in Kerry.
The airport at Kerry has been without a link to Dublin since Ryanair suspended its single commercial flight in September.
The airline also pulled out of the regional subsidised public service obligation route a year ago, blaming high Government charges.
Ryanair had taken over the three-year contract from Aer Arann in 2008.
Aer Arann Chairman, Padraig O’Ceidigh said: “The Kerry Dublin route is a fundamental part of the infrastructure required to promote economic recovery and sustainable growth in the years ahead. This is reflected in the fact that the Government has continued to designate this as a public service obligation route.”
“We all recognise the vital importance of regional air connectivity in general and the key role that this air link to Dublin provides for business, tourism and leisure travel. This was one of the most successful routes in the Aer Arann network and we will work very hard with our partners in Kerry Airport to make it a success again,” he added.
Airport chairman Denis Cregan said passengers will be able to take advantage of the conveniently timed flights which are ideal for both business and leisure.
Mayor of Kerry, Cllr Tim Buckley, County Manager Tom Curran, as well as members of the Oireachtas and Killarney and Tralee Chambers of Commerce were at the airport to show their support for the service.
Flights will be operated by a 48-seater ATR 42 aircraft. Return flights start from €70 including taxes.