The Saints appealed against the red card shown to Mane during Saturday’s 2-1 Premier League victory at Stoke.
Referee Lee Mason gave the forward a straight red card during the first minute of second-half stoppage time when he caught Stoke’s Erik Pieters in mid-air having mistimed a jump to challenge for the ball.
The decision appeared harsh, however, particularly given Mane kept his eyes on the ball, and manager Ronald Koeman indicated the club’s intention to appeal after the game.
“Southampton FC can confirm we have lodged an appeal against the red card shown to Sadio Mane at Stoke on Saturday,” a club statement said yesterday.
Southampton were also unfortunate when Mason denied Dusan Tadic what appeared a penalty following a challenge from Stoke goalkeeper Jack Butland.
Mane’s red card was their sixth of the season, the highest total for any Premier League team.
Meanwhile, Stoke boss Mark Hughes is confident skipper Ryan Shawcross will be fit for Saturday’s Premier League date with Watford at Vicarage Road.
Shawcross returned to action in Saturday’s 2-1 defeat by Southampton after eight matches on the sidelines with damaged ligament in his back.
He stayed the distance but appeared to be struggling in the closing stages but Hughes expected him to be fit to face the Hornets.