The second entry stage provides connections who did not enter at the initial yearling stage the opportunity to add horses to the premier Classic at a cost of £8,000 (€9,691).
The third and final opportunity to enter the Derby comes at the supplementary entry stage on May 28, when the fee rises to £75,000 (€90, 846).
Akeed Mofeed won a Leopardstown maiden last September before finishing a close second in the Beresford Stakes.
The other horses added to the race are the the Aga Khan-owned Kesampour. Trained in France by Mikel Delzangles, the David Lanigan-trained Main Sequence, while Godolphin trainer Mahmood Al Zarooni is responsible for two of the second entries – Mandaean and Swedish Sailor.
Meanwhile French Fifteen confirmed himself a serious Classic contender with an impressive seasonal debut in the Prix Djebel at Maisons-Laffitte yesterday.
The Nicolas Ridden by Olivier Peslier in this seven-furlong Group Three, French Fifteen quickened up smartly to win decisively by a neck.
Abtaal was sent off the hot favourite having beaten French Fifteen comprehensively last year, but the tables were turned.
The second of the major trials on the Maisons-Laffitte card was the Prix Imprudence and this was won easily by Jean-Claude Rouget’s Mashoora under Christophe Soumillon. She is a best priced 16-1 for the Qipco 1000 Guineas at Newmarket on May 6.
lAustralian sensation Black Caviar is only likely to run in the Qipco Sussex Stakes if connections feel it is the right thing to do with the unbeaten mare, regardless of a £1million carrot being dangled for a clash with Frankel.
The big-race sponsors have pledged to up the prize-money for the Group One mile event from its current £300,000 – if Frankel and Black Caviar, the world’s top two horses, turn up at Glorious Goodwood on August 1.
* Joseph O’Brien took the International Jockey Ride of the Year award at the Stobart Lesters at a glittering ceremony at the Hilton Birmingham Metropole last night.
Apprentice Jockey of the Year: Martin Harley
Conditional Jockey of the Year: Sam Twiston-Davies
Flat Ride of the Year: Paul Hanagan – Barefoot Lady, Newmarket
Jump Ride of the Year: Maurice Linehan – Tarvini, Huntingdon
Lady Jockey of the Year: Cathy Gannon
International Jockey Ride of the Year: Joseph O’Brien – Camelot, Doncaster
Jump Jockey Special Recognition: Anna O’Brien (physiotherapist)
Flat Jockey Special Recognition: Philip Robinson
Jump Jockey of the Year: AP McCoy
Flat Jockey of the Year: Paul Hanagan