Sadie, an 18-month-old golden retriever, lived with Kathryn Cuddy and Cathal Ó Sé, who are both vets, in Midleton, Co Cork.
Her owners believe she was stolen last Sunday because her collar with a tag showing she was microchipped was found on the garden gate.
Kathryn and her dog appear in the first episode of Pet Surgeons which airs on RTÉ One next month.
GMarsh TV, makers of Vets on Call, filmed vets treating animals at Gilabbey Veterinary Hospital in Togher, Cork.
Kathryn, one of the 10 vets who work at the hospital, said Sadie was in a secure locked back garden and gardaí were quite satisfied that she had been stolen.
“I have had Sadie since she was eight weeks old. She is a pedigree golden retriever and weighs about 30kg,” she said.
Sadie features in the first episode of the television show when vet Tom Conway neuters the dog using keyhole surgery.
“I was not looking on; I was not anywhere to be seen. I was being kept calm elsewhere in the practice,” said Kathryn.
“I appear in the series; specialise in dermatology, but I would not operate on my own pet.”
Kathryn said Sadie was a little bit timid with new people but warmed to them very quickly.
“We have two cats and a flock of battery chickens. Sadie is best friends with the two cats and she is much loved by us.”
Sadie’s owners have offered a reward to anyone who can help them find her.
Those with information are urged to contact the gardaí.