The DUP Stormont Economy Minister Diane Dodds has attended a meeting with Tánaiste Leo Varadkar.
The North South Trade and Business Development meeting started at 2pm on Tuesday, where it is understood that no items involving the Northern Ireland Protocol were to be discussed.
Stormont Finance Minister Conor Murphy, of Sinn Féin, was also in attendance.
It comes after the DUP were accused last week of boycotting all north/south meetings due to their anger at the post-Brexit trading arrangements.
Unionists and loyalists oppose the Protocol, claiming additional checks on goods places a barrier between Northern Ireland and the rest of the United Kingdom.
Other executive parties held a series of meetings on Monday to discuss two DUP no-shows at sectoral meetings of the North South Ministerial Council in recent weeks.
Sinn Féin president Mary-Lou McDonald said: “Political progress, stability and inclusion relies on all of the institutions of the Good Friday Agreement working, and working well.
“The north-south piece is absolutely essential and that means that unionists of all hues have to participate in those meetings.”
One accompanying minister from the opposing political view must attend North South Ministerial Council meetings to allow them to proceed.
DUP leader Arlene Foster previously said it is “simply not the case” that her party is boycotting north-south political meetings.
She said no ministers from her party had been available to attend recent sectoral meetings on transport and on languages due to timing issues, and a lack of agreement on the agendas.
In February, the DUP warned that north-south relationships will be “impacted” as part of its political campaign to oppose the Northern Ireland Protocol, which governs post-Brexit trading arrangements on the Irish Sea.
The party also pledged to oppose all Protocol-related measures in the Northern Ireland Assembly.
TUV leader Jim Allister accused the DUP of “buckling”.
“Why is a unionist facilitating a meeting to discuss trade and business development on a north-south level when our east-west links in those areas have been trashed by the Protocol?” he said.