Greece's culture ministry has rejected a request by Italian luxury brand Gucci to use the ancient Acropolis for a fashion event this summer.
Culture Minister Lydia Koniordou told state television a panel of ministry experts turned down the request, adding she was in "total agreement".
The Acropolis is listed as a World Heritage Site by the United Nations cultural organisation, Unesco.
Ms Koniordou said: "We have a duty to defend the importance of (the Acropolis) ... a global symbol of democracy and freedom."
Requests for commercial use of ancient monuments in Greece are rarely approved.
American singer Jennifer Lopez was granted access to the Acropolis for a 2008 photo shoot.
The site also featured in the 2014 film The Two Faces Of January starring Viggo Mortensen and Kirsten Dunst.