Robbie Keane scored twice for LA Galaxy but his efforts were in vain as his side were dealt a 5-2 shock defeat by the Portland Timbers.
The Galaxy would have secured a semi-final spot in Major League Soccer's Western Conference semi-finals with a game to spare had they won.
The first goal for the Republic of Ireland captain was a superb volley into the top corner from a long ball out of the Galaxy defence.
His second was a header flicked into the net from a cross by Jose Villarreal, but it wasn't enough to stop LA from losing to an impressive Portland side.
Fanendo Ali also netted twice for the Timbers, with Diego Chara, Darlington Nagbe and Maximiliano Urruti joining him on the scoresheet for Portland.
They now stand fourth in the Western Conference, close to tying up their post-season place ahead of the final full round of games.
Mike Grella scored the fastest goal in Major League Soccer history when his strike after seven seconds set the New York Red Bulls on their way to a 4-1 win over the Philadelphia Union.
New York secured top seeding for the Eastern Conference play-offs with their victory, which was never in doubt. Bradley Wright-Phillips doubled the home team’s lead after four minutes, Grella scored his second and Kemar Lawrence made it a first-half rout when putting New York 4-0 ahead just before the break.
Sebastien Le Toux scored a 55th-minute consolation for Philadelphia.
Former Leeds midfielder Grella’s landmark goal came from the Union’s kick-off.
Cristian Maidana laid the ball back to team-mate Michael Lahoud who failed to read his intentions, and Grella reached it first, sprinting at goal before his left-footed shot took a deflection off defender Steven Vitoria and nestled in the bottom left corner.
The goal beat Tim Cahill’s eight-second goal for the Red Bulls against the Houston Dynamo in October 2013.
DC United beat the Chicago Fire 4-0, to set up a showdown next week with the Columbus Crew when second place in the Eastern Conference will be on the line. Chris Pontius, Bobby Boswell, Fabian Espindola and Alvaro Saborio scored, to nudge DC United one point ahead of Columbus.
The Houston Dynamo’s play-off hopes were ended by a 1-1 home draw against the Seattle Sounders.
Houston edged ahead when Ricardo Clark scored in the 65th minute, but Seattle striker Obafemi Martins levelled after 82 minutes.
Seattle stand fifth but will slip a place if Sporting Kansas City avoid defeat to the Colorado Rapids tonight.