Unionists who voted to remain in the EU were not voting to leave the UK, UUP leader Robin Swann has told Michel Barnier.
Mr Swann and Ulster Unionist MEP Jim Nicholson met the EU's chief Brexit negotiator in Strasbourg this morning.
The UUP backed the Remain campaign ahead of the 2016 referendum, in which Northern Ireland as a region voted 56% to 44% to stay within the EU.
The party's position since the vote is that the overall result should be respected and Brexit implemented.
It has reacted angrily to proposals from the EU that would see Northern Ireland treated differently to the rest of the UK in order to maintain a free-flowing border on the island of Ireland.
Mr Swann said the discussions with Mr Barnier were "constructive and business-like".
"We were keen to address a number of the misconceptions currently being peddled by some," he said.
"It was a democratic vote which all unionists respect. Unionists are unionists for a reason.
"The tone of the language being used by some, more specifically the Irish government, is less than helpful.
"The Irish Government in particular seem intent on using Brexit as a Trojan Horse to pursue Irish Unity by other means."
Mr Swann accused the Irish Government of undermining the key principle of the Good Friday Agreement - that it is for the people of Northern Ireland to decide their constitutional future.
He added: "We also told Mr Barnier that Northern Ireland welcomed the help and assistance of the EU over the years through its peace programmes.
"It is widely recognised that the EU did great work in this area, but that good work is now being undermined because there is a growing perception that the EU is now siding with nationalists against unionists.
"We invited Mr Barnier to come to Northern Ireland and speak directly to unionists to get their views."