Dublin Bus has confirmed services will terminate tomorrow night at 9pm, ahead of a second two-day strike by drivers on Thursday and Friday.
The company says the move is to ensure the safe return of buses to their depots.
Dublin Bus says each day of industrial action is costing the company more than €600,000.
It claims this will make it more difficult to fund the original 8.25% pay rise recommended by the Labour Court.
Meanwhile, the trade union Siptu has accused the company, the Department and the Minister for Transport of "complete intransigence" in relation to its pay claim.
Siptu's John Murphy, said: "The complete intransigence shown by Dublin Bus, the Department and the Minister for Transport in response our members need for an acceptable pay rise, has created real anger and frustration.
"The fact that the work stoppage on Friday will also impact on Culture Night in Dublin is regrettable.
"Siptu representatives will meet with their colleagues in the four other trade unions in Dublin Bus on Thursday to discuss further industrial action. Our members deeply regret they are being forced into a situation where they may have to further disrupt this vital public service and the lives of those that rely on it.
"Siptu representatives have repeatedly stated we are available to enter negotiations to resolve this dispute but this has not been reciprocated by management or the Department of Transport."
The NBRU has also urged Dublin Bus management to return to the negotiating table.
General Secretary Dermot O'Leary said: "The 400,000 bus commuters in Dublin deserve more than silence from Official Ireland. There has been absolutely no contact, either directly or indirectly, with staff representatives from the Minister's Office, Department of Transport Officials, the National Transport Authority or those in the CIÉ Group since the commencement of this dispute.
"Such a state of affairs is simply not good enough, playing foot loose and fancy free with a vital public service which underpins the economic and social fabric of Dublin is intolerable and suggests that those directly charged with its provision are devoid of the ability to work towards a solution.
"This dispute will only be resolved around the negotiating table, of all the options that may be available, sitting on one's hands is surely not one of them."
"We as representatives of the workers also have an equal responsibility to work towards a resolution to such disputes, traditionally with the assistance of the State's industrial relations institutions. We remain available to play our part."