Staff in Childminding Ireland are threatening to begin indefinite strike action.
They want management to withdraw compulsory redundancy notices and engage with staff over proposed restructuring plans.
In a statement, the Impact trade union said that the organisation is not experiencing financial difficulties and has produced no business case to justify redundancies.
The union said that it believes Childminding Ireland is illegally threatening redundancies to force staff out of their jobs before replacing them with others.
Five of six staff members have voted to take industrial action from June 7 - the union said that this gives management enough time to resolve the issue at the Workplace Relations Commission.