The ASTI, which represents second level teachers, says they will hold two separate ballots on industrial action.
The announcement follows today's meeting of the members' central executive council in Dublin.
Members will be balloted on industrial action - up to and including strike action - over new teachers' pay.
Teachers who entered the profession after 2010 are on different pay scales than their colleagues even though they carry out the same duties and responsibilities.
In a separate ballot which relates to measures applied to ASTI members following their rejection of the Lansdowne Road Agreement, members will be asked to vote on withdrawal from supervision and substitution duties.
At today's meeting the ASTI also decided to hold a protest over reforms to the Junior Cycle.
The union is currently undertaking industrial action on the issue, to highlight their concerns which include the lack of ordinary and higher level options in most subjects.
The ASTI say they have a mandate to escalate the measure up to strike action.