'Taggart' has been axed by ITV bosses after 28 years on air.
The police show - which is the UK's longest running crime drama - has been cancelled after 110 episodes because viewing figures decreased from 14 million in its heyday to 3.8 million during the last series.
A message on the programme's website reads: "This programme is now over."
The Glasgow-based show focused on the detective work of DCI Jim Taggart - played by the late Mark McManus until his death in 1994 - who was famous for his "There's been a murder" catchphrase.
He was replaced by DI Mike Jardine, who was played by James MacPherson.
The show - which has featured cameo appearances from the likes of Robert Carlyle and Annette Crosbie - narrowly escaped the axe in 2009 when it received 5.2 million viewers.
However, ITV bosses have now decided to pull the plug on the show to make way for "new and original drama".
An ITV spokesman confirmed: "Since it was first broadcast in 1983, 'Taggart' has been one of ITV's most enduring dramas. But, reflecting the demands of our audience and as part of the ongoing creative renewal of the ITV1 schedule, our priority is to invest in new and original drama."
Stars of the last series include John Michie, who played DI Robert Ross, Bylthe Duff (DI Jackie Reid) and Alex Norton (DCI Matt Burke).