Tobey Maguire and Jennifer Meyer have separated after nine years of marriage.
Maguire's representative confirmed a People magazine report about the break-up.
The pair told People in a joint statement that the decision came "after much soul searching and consideration".
Maguire and Meyer said their "first priority remains raising our children together with enduring love, respect and friendship".
They have a nine-year-old daughter, Ruby, and a seven-year-old son, Otis.
Meyer is a jewellery designer and the daughter of long-time Hollywood executive Ron Meyer.